Transformation with Artificial Chromosomes in Oxytricha trifallax and Their Applications
نویسندگان
چکیده
منابع مشابه
The Oxytricha trifallax Mitochondrial Genome
The Oxytricha trifallax mitochondrial genome contains the largest sequenced ciliate mitochondrial chromosome (~70 kb) plus a ~5-kb linear plasmid bearing mitochondrial telomeres. We identify two new ciliate split genes (rps3 and nad2) as well as four new mitochondrial genes (ribosomal small subunit protein genes: rps- 2, 7, 8, 10), previously undetected in ciliates due to their extreme divergen...
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The macronuclear genome of the ciliate Oxytricha trifallax displays an extreme and unique eukaryotic genome architecture with extensive genomic variation. During sexual genome development, the expressed, somatic macronuclear genome is whittled down to the genic portion of a small fraction (∼5%) of its precursor "silent" germline micronuclear genome by a process of "unscrambling" and fragmentati...
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We propose construction of a BAC library for the germline micronuclear genome (MIC) of the ciliate protist Oxytricha trifallax (recently renamed Sterkiella histriomuscorum; alveolate; class Spirotrichia). This library will primarily be used as a tool to characterize the structure and function of the ciliate micronucleus and its relationship to that of the somatic macronucleus, rather than for c...
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Ciliates have two different types of nuclei per cell, with one acting as a somatic, transcriptionally active nucleus (macronucleus; abbr. MAC) and another serving as a germline nucleus (micronucleus; abbr. MIC). Furthermore, Oxytricha trifallax undergoes extensive genome rearrangements during sexual conjugation and post-zygotic development of daughter cells. These rearrangements are necessary b...
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ژورنال
عنوان ژورنال: G3 Genes|Genomes|Genetics
سال: 2019
ISSN: 2160-1836
DOI: 10.1534/g3.119.400298